Laboratory of Plant Biotechnology (UALG)

We conduct research on the use of biotechnological approaches towards the conservation and sustainable use of plant genetic resources and on the biological and chemical characterization of natural compounds from plant origin.

Team

Anabela Maria Lopes Romano

Head

Anabela Maria Lopes Romano

Alfredo Jaime Morais Cravador

Researcher

Alfredo Jaime Morais Cravador

Raquel Rodríguez Solana

Researcher

Raquel Rodríguez Solana

Natacha Rodrigues Coelho

Researcher

Natacha Rodrigues Coelho

María José Aliaño González

Researcher

María José Aliaño González

José M. P. T. Leitão

Researcher

José M. P. T. Leitão

Hugo Manuel Matias Duarte

Researcher

Hugo Manuel Matias Duarte

Bruno Filipe Figueiras Medronho

Researcher

Bruno Filipe Figueiras Medronho

Sandra Marisa Gomes Gonçalves

Researcher

Sandra Marisa Gomes Gonçalves

Inês Filipa Paixão Mansinhos

Researcher - PhD Student

Inês Filipa Paixão Mansinhos

Contacts

Universidade do Algarve, Faculdade de Ciências e Tecnologia,
Campus de Gambelas, Ed. 8, lab. 3.43 8005-139 Faro, Portugal

Gabinete: +351 289 800 910 Lab: +351 289 800 900 Ext.: 7323*

aromano@ualg.pt

* Call cost to Portugal landline

Research

The research taking place at the Plant Biotechnology Laboratory of the University of Algarve falls into the following main fields:

Plant tissue culture: in this research line, we use plant tissue culture techniques for the propagation of wild endangered species, aromatic/medicinal and plants of agronomic interest. So far, in vitropropagation protocols have been developed for several species, such as Ceratonia siliqua, Drosera intermedia, Drosophyllum lusitanicum, Lavandula viridis, Pinguicula lusitanica, Thymus lotocephalus, Tuberaria globularifolia var. major, among others. These methodologies allow the propagation of uniform plants in a controlled environment, independently of seasonal variations, and, therefore, have been a useful tool for other studies, namely: i) to study the physiological and (bio)chemical response of species of the Lamiaceae family to abiotic stress conditions (drought, temperature, salinity and UVB radiation) with special emphasis on the production of phenolic compounds and essential oils; and ii) to evaluate the effectiveness of manipulating the composition of the culture medium and of elicitation in increasing the production of phenolic compounds of industrial interest aiming their sustainable production.

Germplasm conservation: worldwide, many plant species are threatened with extinction especially due to various human activities. Yet plants are part of our natural heritage, and it is our responsibility to preserve and protect them for future generations. The application of conservation approaches based on biotechnology has given a major contribution to plant conservation. The purpose is not the replacement of traditional conservation methods but to complement and improve the methods already available. Biotechnology can provide tools not only for germplasm conservation but also for its management and utilization. The field of application ranges from the conservation of endangered wild species to the storage of genotypes or genetically modified material of economically important species. At the Plant Biotechnology Laboratory, we have contributed to the ex situ conservation of several wild species in the Algarve region, including endemic and endangered species, using different biotechnological tools. In addition to developing numerous in vitro propagation protocols based on tissue culture, we also carry out cryopreservation experiments. Cryopreservation is a type of long-term conservation and can be applied to different types of tissues, including seeds, shoot tips, or nodal segments. Seeds from Thymus lotocephalus, Tuberaria globularifolia var. major and Plantago algarbiensis, endemic and rare species from the Algarve, were successfully cryopreserved. We developed protocols for cryopreservation of shoot tips (T. lotocephalus and T. globularifolia), and nodal segments (P. algarbiensis), using the cryopreservation methods vitrification, droplet-vitrification and encapsulationdehydration. More recently, we have begun to test the feasibility of using natural deep eutectic solvents (NADES) as a substitute for conventional cryoprotective solutions used in vitrification-based methods, which are often toxic to plant cells. NADES could be a green and less harmful alternative to plant tissues, contributing to the development of new cryopreservation strategies not only for the aforementioned species but also for other vulnerable species. In addition to micropropagation and cryopreservation, we also carry out genetic diversity studies of populations of these species, which may contribute to the creation of effective conservation strategies, preventing their decline.

Chemical and biological characterization of matrices from wild plants and agro-food products: given the growing demand for natural products, mainly of plant origin, and the importance of Mediterranean flora valorization, this research line aims at optimizing processes for extracting bioactive compounds from plant matrices, giving priority to the use of green methodologies and solvents, and the subsequent phytochemical and bioactivity study of the extractables obtained. In this context, we prioritize the study of edible and/or medicinal wild plants and traditional agri-food products from the Algarve,and the use of natural deep eutectic solvents (NADES) as an alternative to more toxic organic solvents. Phytochemical studies include mainly the analysis of the phenolic and/or volatile profile. The evaluation of bioactivity includes several in vitro tests, with the aim of evaluating the medicinal or cosmetic potential (antioxidant, enzyme inhibitor, photoprotector) of the obtained extracts/products.Combined studies of Phlomis purpurea metabolome and transcriptome to unveil mechanisms underlying the auto-immunity of this plant to the devastating pathogen Phytophthora cinnamomi.In addition to understanding the involvement of secondary metabolites in the defenseof P. purpurea it is expected to disclose new substances with potential therapeutic effects as a profusion of secondary metabolites from this genus has been isolated and identified, some of them presenting pharmacological activity.Study of Phlomis purpurea potential as an antagonist for phytopathogens biological control aiming at the implementation of a safer strategy than the use of fungicides or other hazardous molecules to prevent root rot of crops or forest species.Field studies in cork oak forest to assess the capacity of Phlomis purpurea to eradicate Phytophthora cinnamomi.

Handling and valuing renewable resources: the extraction and dissolution of value-added compounds (such as polyphenols) and natural biopolymers (such as cellulose, lignin, chitin, locust bean gum and fibroin) arehighly relevant in the context of valuing agroforestry residues. Our work has essentially focused on the development of new solvents, such as binary and ternary eutectic systems. Systems consisting of cations and anions of biological origin, such as those obtained from natural organic acids, amino acids, non-nutritive sweeteners or natural compounds, such as choline or betaine, have been explored. This research line also seeks to understand the fundamental aspects (intermolecular interactions) that determine or affect the extraction and/or dissolution performance of a given solvent. The regeneration (precipitation, gelation) of biopolymers, the mechanisms involved and the development of new materials, such as composites, films, foams, resins and particles are other important aspects of the research line. In this context, we have sought to develop films for applications both in packaging and as triboelectric materials for energy harvesting. On the other hand, the developed foams have been explored as matrices for removing polluting agents from aqueous media. New functionalities of the biopolymers are also envisaged, with the aim of increasing their versatility and applicability. New cellulose and lignin derivatives have been developed (tuning the charge density and degree of hydrophobicity) with the objective of their incorporation in cosmetic formulations (hair conditioners) or as flocculating agents for microplastics or even for the development of new hydrogels for agricultural applications with superior absorption capacity and controlled release of water, fertilizers and phytochemicals. The laboratory also has equipment for the mechanical and optical rheological characterization of several systems with very different properties (from viscous fluids to elastic solids)

Publications

2023

Delgado A., Gonçalves S., Romano A., 2023. Mediterranean Diet: the role of phenolic compounds from aromatic plant foods. Foods, 12, 840. https://doi.org/10.3390/foods12040840

Medronho B., Pereira A., Duarte H., Gentile L., Costa A., Romano A., Olsson U., 2023. Probing cellulose–solvent interactions with self-diffusion NMR: Onium hydroxide concentration and co-solvent effects. Carbohydrate Polymers, 303, 120440. https://doi.org/10.1016/j.carbpol.2022.120440

Erazo Solorzano, C. Y., Disca, V., Muñoz-Redondo, J. M., Tuárez García, D. A., Sánchez-Parra, M., Carrilo Zenteno, M. D., Moreno-Rojas, J. M., Rodríguez-Solana, R., 2023. Effect of drying technique on the volatile content of Ecuadorian Bulk and Fine-Flavor cocoa. Foods12(5), 1065. https://doi.org/10.3390/foods12051065

Muñoz-Redondo, J. M., Puertas, B., Valcárcel-Muñoz, M. J., Rodríguez-Solana, R., Moreno-Rojas, J.M., 2023. Impact of stabilization method (traditional versus cold) and filtration step on the ester profile of “Brandy de Jerez”. A chemometric assessment. Applied Science. Accepted. https://doi.org/10.3390/app13063428

Gutiérrez-Escobar, R.; Aliaño-González, M.J.; Marrufo-Curtido, A.; Jiménez-Hierro, M.J.; Puertas, B.; Cantos-Villar, E., 2023. Sulfur Dioxide-Free Verdejo Wines through the Use of a Pure Stilbene Extract: Exploring Possible Synergistic Effect with Glutathione. Journal of the Science of Food and Agriculture, 103, 1152–1160. https://doi.org/10.1002/jsfa.12209

2022

Mansinhos I., Gonçalves S., Rodríguez Solana R., Ordóñez-Díaz J.L., Moreno-Rojas J.M., Romano A., 2022. Impact of Temperature on Phenolic and Osmolyte Contents in In Vitro Cultures and Micropropagated Plants of Two Mediterranean Plant Species, Lavandula viridis and Thymus lotocephalus. Plants, 11, 3516. https://doi.org/10.3390/plants11243516

Duarte H., Gomes V., Aliaño-González M.J., Medronho B., Faleiro F. & Romano A., 2022. Ultrasound–Assisted Extraction of Polyphenols from Maritime Pine Residues with Deep Eutectic Solvents. Foods, 11(23), 3754. https://doi.org/10.3390/foods11233754

Duarte H., Gummel J., Robles E., Berti D. & Fratini E., 2022. Ultra-/Small Angle X-ray Scattering (USAXS/SAXS) and Static Light Scattering (SLS) Modeling as a Tool to Determine Structural Changes and Effect on Growth in S. epidermidis. ACS Appl. Bio Mater. https://doi.org/10.1021/acsabm.2c00218

Barea-Sepúlveda M., Duarte H., Aliaño-González M.J., Romano A. & Medronho B., 2022. Total Ion Chromatogram and Total Ion Mass Spectrum as Alternative Tools for Detection and Discrimination (A Review). Chemosensors 2022, 10, 465. (Selected by the editors as the issue cover). https://doi.org/10.3390/chemosensors10110465

Mansinhos I., Gonçalves S., Rodríguez Solana R., Duarte H., Ordóñez-Díaz J.L., Moreno-Rojas J.M., Romano A., 2022. Response of Thymus lotocephalus In Vitro Cultures to Drought Stress and Role of Green Extracts in Cosmetics. Antioxidants 11, 1475. https://doi.org/10.3390/antiox11081475

Baca-Bocanegra B., Gonçalves S., Nogales-Bueno J., Mansinhos I., Heredia F.J., Hernández-Hierro J.M., Romano A., 2022. Influence of wine pH and ethanol content on the fining efficacy of proteins from winemaking byproducts. Foods 11, 1688. https://doi.org/10.3390/foods11121688

Magalhães S., Alves L., Romano A., Medronho B., Rasteiro, M.G., 2022. Extraction and characterization of microplastics from Portuguese industrial effluents. Polymers, 14, 2902. https://doi.org/10.3390/polym14142902

Magalhães S., Moreira A., Almeida R., Cruz P., Alves L., Costa C., Mendes C., Medronho B., Romano A., Carvalho M.G., Gamelas J., Rasteiro, M.G., 2022. Acacia wood fractionation using deep eutectic solvents: extraction, recovery and characterization of the different fractions. ACS Omega, 7, 30, 26005–26014. https://doi.org/10.1021/acsomega.1c07380

Melro E., Antunes F.E., Valente A.J.M., Duarte H., Romano A., Medronho B., 2022. On the development of phenol-formaldehyde resins using a new type of lignin extracted from pine wood with a levulinic-acid based solvent. Molecules, 27, 27(9):2825. https://doi.org/10.3390/molecules27092825

Das G., Gonçalves S.,  Heredia J.B., Romano A.,  Jiménez-Ortega L.A., Gutiérrez-Grijalva E.P.,  Shin H.S., Patra J.K., 2022. Cardiovascular protective effect of cinnamon and its major bioactive constituents: An update. Journal of Functional Foods, pp. 105045. https://doi.org/10.1016/j.jff.2022.105045

Aliaño-González, M.J.; Gabaston, J.; Ortiz-Somovilla, V.; Cantos-Villar, E., 2022. Wood Waste from Fruit Trees: Biomolecules and Their Applications in Agri-Food Industry. Biomolecules, 12, 238. https://doi.org/10.3390/biom12020238

Muñoz-Redondo, J. M., Valcárcel-Muñoz, M. J., Solana, R. R., Puertas, B., Cantos-Villar, E., & Moreno-Rojas, J. M., 2022. Development of a methodology based on headspace solid-phase microextraction coupled to gas chromatography-mass spectrometry for the analysis of esters in brandies. Journal of Food Composition and Analysis108, 104458. https://doi.org/10.1016/j.jfca.2022.104458

Kaur, I., Sharma, A. D., Samtiya, M., Pereira-Caro, G., Rodríguez-Solana, R., Dhewa, T., & Moreno-Rojas, J. M., 2022. Potential of bioactive compounds derived from underutilized fruit-bearing plants: a comprehensive review. European Food Research and Technology, 1-20. https://doi.org/10.1007/s00217-022-04171-z

Sánchez-Parra, M., Lopez, A., Muñoz-Redondo, J. M., Montenegro-Gómez, J. C., Pérez-Aparicio, J., Pereira-Caro, G., Rodríguez-Solana, R., Moreno-Rojas, J. M. & Ordóñez-Díaz, J. L., 2022. Study of the influence of the fishing season and the storage temperature in the fishing vessel on the biogenic amine and volatile profiles in fresh yellowfin tuna (Thunnus albacares) and dry-cured mojama. Journal of Food Composition and Analysis114, 104845. https://doi.org/10.1016/j.jfca.2022.104845

Barea-Sepúlveda, M.; Duarte, H.; Aliaño-González, M.J.; Romano, A.; Medronho, B., 2022. Total Ion Chromatogram and Total Ion Mass Spectrum as Alternative Tools for Detection and Discrimination (A Review). Chemosensors, 10, 465. https://doi.org/10.3390/chemosensors10110465

Calle, J.L.P.; Falatová, B.; Aliaño-González, M.J.; Ferreiro-González, M.; Palma, M., 2022. Machine Learning Approaches over Ion Mobility Spectra for the Discrimination of Ignitable Liquids Residues from Interfering Substrates. Talanta Open, 6, 100125. https://doi.org/10.1016/j.talo.(2022).100125

Alavarsa-Cascales, D.; Aliaño-González, M.J.; Palma, M.; Barbero, G.F.; Carrera, C., 2022. Optimization of an Enzyme-Assisted Extraction Method for the Anthocyanins Present in Açai (Euterpe Oleracea Mart.). Agronomy, 12, 2327. https://doi.org/10.3390/agronomy12102327

Aliaño-González, M.J.; Barea-Sepúlveda, M.; Espada-Bellido, E.; Ferreiro-González, M.; López-Castillo, J.G.; Palma, M.; Barbero, G.F.; Carrera, C., 2022. Ultrasound-Assisted Extraction of Total Phenolic Compounds and Antioxidant Activity in Mushrooms. Agronomy, 12, 1812. https://doi.org/10.3390/agronomy12081812

Aliaño-González, M.J.; Montalvo, G.; García-Ruiz, C.; Ferreiro-González, M.; Palma, M., 2022. Assessment of Volatile Compound Transference through Firefighter Turnout Gear. International Journal of Environmental Research and Public Health, 19, 3663. https://doi.org/10.3390/ijerph19063663

Duarte, H.; Gomes, V.; Aliaño-González, M.J.; Faleiro, L.; Romano, A.; Medronho, B., 2022. Ultrasound-Assisted Extraction of Polyphenols from Maritime Pine Residues with Deep Eutectic Solvents. Foods, 11, 3754. https://doi.org/10.3390/foods11233754

Aliaño-González, M.J.; Carrera, C.; Barbero, G.F.; Palma, M., 2022. A Comparison Study between Ultrasound–Assisted and Enzyme–Assisted Extraction of Anthocyanins from Blackcurrant (Ribes Nigrum L.). Food Chemistry: X, 13, 100192. https://doi.org/10.1016/j.fochx.2021.100192

Piñeiro, Z.; Aliaño-González, M.J.; González-de-Peredo, A.V.; Palma, M.; de Andrés, M.T., 2022. Microwave-Assisted Extraction of Non-Coloured Phenolic Compounds from Grape Cultivars. European Food Research Technology, 248, 1883–1901. https://doi.org/10.1007/s00217-022-04013-y

Vázquez-Espinosa, M.; González-de-Peredo, A.V.; Carrera, C.; Palma, M.; Barbero, G.F.; Aliaño-González, M.J., 2022. Ultrasound-Assisted Extraction of Betalains from Opuntia Fruit Pulp of Different Color Varieties. Agronomy, 12, 2604. https://doi.org/10.3390/agronomy12112604

Maia, C., Horta Jung, M., Carella, G., .Moricca, S., Jung, T., 2022. Eight new Halophytophthora species from marine and brackish-water ecosystems in Portugal and an updated phylogeny for the genus. Persoonia: Molecular Phylogeny and Evolution of Fungi, 48, 54–90. https://doi.org/10.3767/persoonia.2022.48.02

Reis, J.M., Pereira, R.J., Coelho, P.S., Leitão, J.M., 2022. Assessment of Wild Rocket (Diplotaxis tenuifolia (L.) DC.) Germplasm Accessions by NGS Identified SSR and SNP Markers. Plants, 11(24), 3482. https://doi.org/10.3390/plants11243482

Coelho, P.S., Reis, J.M., Pereira, A.L., Lopes, V., Leitão, J.M., 2022. Downy mildew resistance and genetic variability in a wild rocket germplasm collection. Agronomy Journal, 114(6), 3083–3095. https://doi.org/10.1002/agj2.21190

2021

Coelho N., Romano A., 2021. Impact of chitosan on plant tissue culture: recent applications. Plant Cell, Tissue and Organ Culture 148: 1–13. https://doi.org/10.1007/s11240-021-02156-6

Costa, Carolina; Medronho, Bruno; Eivazi, Alireza; Svanedal, Ida; Lindman, Björn; Edlund, Håkan; Norgren, Magnus., 2021. Lignin enhances cellulose dissolution in cold alkali. Carbohydrate Polymers, 274, 118661. https://doi.org/10.1016/j.carbpol.2021.118661

Alireza Eivazi; Bruno Medronho; Björn Lindman; Magnus Norgren., 2021. On the Development of All-Cellulose Capsules by Vesicle-Templated Layer-by-Layer Assembly. Polymers, 13, 589-589. https://doi.org/10.3390/polym13040589

Björn Lindman; Bruno Medronho; Luís Alves; Magnus Norgren; Lars Nordenskiöld., 2021. Hydrophobic interactions control the self-assembly of DNA and cellulose. Quarterly Reviews of Biophysics, 54https://doi.org/10.1017/S0033583521000019

Costa C., Medronho B., Filipe A., Romano A., Lindman B., Edlund H., Norgren M., 2021. On the formation and stability of cellulose-based emulsions in alkaline systems: effect of the solvent quality. Carbohydrate Polymers. https://doi.org/10.1016/j.carbpol.2022.119257

Pan Y.-L., Rodrigues M.J., Pereira C.G., Engrola S., Colen R., Mansinhos I., Romano A., Andrade P., Fernandes F., Custódio L., 2021. Exploring the biotechnological value of marine invertebrates: a closer look at the biochemical and antioxidant properties of Sabella spallanzanii and Microcosmus squamiger. Animals, 11(12): 3557. https://doi.org/10.3390/ani11123557

Gonçalves S., Mansinhos I., Rodríguez Solana R., Pereira-Caro G., Moreno-Rojas J.M., Romano A., 2021. Impact of metallic nanoparticles on in vitro culture, phenolic profile and biological activity of two Mediterranean Lamiaceae species: Lavandula viridis L’Hér and Thymus lotocephalus G. López and R. Morales. Molecules, 26, 6427. https://doi.org/10.3390/molecules26216427

Rukavina I, Rodrigues M.J., Pereira C.G., Mansinhos I., Romano A., Ślusarczyk S., Matkowski A., Custódio L., 2021. Greener is better: first approach to use natural deep eutectic solvents (NADES) to extract antioxidants from the medicinal halophyte Polygonum maritimum L. Molecules, 26: 6136. https://doi.org/10.3390/molecules26206136

Mansinhos I., Gonçalves S., Rodríguez Solana R., Ordóñez-Díaz J.L., Moreno-Rojas J.M., Romano A., 2021. Ultrasonic-Assisted Extraction (UAE) and NADES combination: A green strategy to improve the recovery of phenolic compounds from Lavandula pedunculata subsp. lusitanica (Chaytor) Franco. Antioxidants, 10, 582. https://doi.org/10.3390/antiox10040582

Rodríguez-Solana R., Romano A., Moreno-Rojas J.M., 2021. Carob Pulp: A Nutritional and Functional By-Product Worldwide Spread in the Formulation of Different Food Products and Beverages. A Review. Processes, 9, 1146. https://doi.org/10.3390/pr9071146

Coelho N., Filipe A., Medronho B., Magalhães S., Vitorino C., Alves L., Gonçalves S., Romano A., 2021. Rheological and microstructural features of plant culture media doped with biopolymers: influence on the growth and physiological responses of in vitro grown shoots of Thymus lotocephalus. Polysaccharides, 2, 538–553. https://doi.org/10.3390/polysaccharides2020032

Das G., Shin H.-S., Tundis R., Gonçalves S., Ourlad Alzeus G. Tantengco, Acquaviva, Campos M., Acquaviva R., Malfa G.A., Romano A., Robles J.A.H., Clores M.Q., Patra J.K., 2021. Plant species of sub-family Valerianaceae: a review on its effect on the central nervous system. Plants, 10, 846, Review paper. https://doi.org/10.3390/plants10050846

Melro E., Filipe A., Sousa D., Medronho B. & Romano A., 2021. Revisiting lignin: a tour through its structural features, characterization methods and applications. New Journal of Chemistry, 45: 6986 - 7013 (Cover). https://doi.org/10.1039/D0NJ06234K

Melro E., Valente A.J.M, Antunes F.E., Romano A., Medronho B., 2021. Enhancing lignin dissolution and extraction: The effect of surfactants. Polymers, 13, 714. https://doi.org/10.3390/polym13050714

Magalhães S., Filipe A., Fernandes C., Melro E., Vitorino C., Alves L., Romano A., Rasteiro M.A. & Medronho B., 2021. Lignin extraction from waste pine sawdust using biomass derived solvent system. Polymers, 13(7), 1090. https://doi.org/10.3390/polym13071090

Fernandes C., Melro E., Magalhães S., Alves L., Craveiro R., Filipe A., Valente A.J.M., Martins G., Antunes F.E., Romano A., Medronho B., 2021. New deep eutectic solvent assisted extraction of highly pure lignin from maritime pine sawdust (Pinus pinaster). International Journal of Biological Macromolecules, 177: 294-305. https://doi.org/10.1016/j.ijbiomac.2021.02.088

Djaoudenea O., Mansinhos I., Gonçalves S., Jara-Palacios M.J., Bachir M.b., Romano A.,2021. Phenolic profile, antioxidant activity and enzyme inhibitory capacities of fruit and seed extracts from different Algerian cultivars of date (Phoenix dactylifera L.) were affected by in vitro simulated gastrointestinal digestion. South African Journal of Botany, 137: 133-148. https://doi.org/10.1016/j.sajb.2020.10.015

 Costa C., Rosa P., Filipe A., Medronho B., Romano A., Liberman L., Talmon Y., Norgren M., 2021. Cellulose-stabilized oil-in-water emulsions: Structural features, microrheology, and stability. Carbohydrate Polymers, 252: 117092. https://doi.org/10.1016/j.carbpol.2020.117092

Alves L., Medronho B., Filipe A., Romano A., Rasteiro M.G., Lindman B., Topgaard D., Davidovich I., Talmon Y., 2021. Revisiting the dissolution of cellulose in H3PO4 (aq.) through cryo-TEM, PTssNMR and DWS.  Carbohydrate Polymers, 252: 117122. https://doi.org/10.1016/j.carbpol.2020.117122

Rodríguez-Solana R., Esteves E., Mansinhos I., Gonçalves S., Pérez-Santín E., Galego L. & Romano A., 2021. Influence of elaboration process on chemical, biological and sensory characteristics of European pennyroyal liqueurs. Journal of the Science of Food and Agriculture, 101: 4076-4089. https://doi.org/10.1002/jsfa.11043

Perez Santin, E., Rodríguez Solana, R., González García, M., García Suárez, M. D. M., Blanco Díaz, G. D., Cima Cabal, M. D., Moreno Rojas, J. M. & *Lopez Sanchez, J. I., 2021. Toxicity prediction based on artificial intelligence: A multidisciplinary overview. Wiley Interdisciplinary Reviews: Computational Molecular Science11(5), e1516. https://doi.org/10.1002/wcms.1516

Barreiro, R., Rodríguez-Solana, R., Alonso, L., Salinero, C., López Sánchez, J. I., & *Pérez-Santín, E., 2021. Fast 1H-NMR Species Differentiation Method for Camellia Seed Oils Applied to Spanish Ornamentals Plants. Comparison with Traditional Gas Chromatography. Plants10(10), 1984. https://doi.org/10.3390/plants10101984

Carrera, C.; Aliaño-González, M.J.; Rodríguez-López, J.; Ferreiro-González, M.; Ojeda-Copete, F.; Barbero, G.F.; Palma, M., 2021. Optimization of an Ultrasound-Assisted Extraction Method for the Analysis of Major Anthocyanin Content in Erica Australis Flowers. Molecules, 26, 2884. https://doi.org.10.3390/molecules26102884

Carrera, C.; Aliaño-González, M.J.; Valaityte, M.; Ferreiro-González, M.; Barbero, G.F.; Palma, M., 2021. A Novel Ultrasound-Assisted Extraction Method for the Analysis of Anthocyanins in Potatoes (Solanum Tuberosum L.). Antioxidants, 10, 1375. https://doi.org10.3390/antiox10091375

Gutiérrez-Escobar, R.; Aliaño-González, M.J.; Cantos-Villar, E., 2021. Wine Polyphenol Content and Its Influence on Wine Quality and Properties: A Review. Molecules, 26, 718. https://doi.org/10.3390/molecules26030718

Jaén-González, L.; Aliaño-González, M.J.; Ferreiro-González, M.; Barbero, G.F.; Palma, M., 2021. A Novel Method Based on Headspace-Ion Mobility Spectrometry for the Detection and Discrimination of Different Petroleum Derived Products in Seawater. Sensors, 21, 2151. https://doi.org/10.3390/s21062151

Pereira, R., Leitão, J.M., 2021. A non-rogue mutant line induced by ENU mutagenesis in paramutated rogue peas (Pisum sativum l.) is still sensitive to the rogue paramutation. Genes, 12(11), 1680. https://doi.org/10.3390/genes12111680

2020

Das G., Lim K.J., Tantengco O.A.G., Carag H.M., Gonçalves S., Romano A., Das S.K., Coy-Barrera E., Shin H.-S. Gutiérrez-Grijalva E.P., Heredia J.B. & Patra J.K., 2020. Cactus: chemical, nutraceutical composition and potential bio-pharmacological properties. Phytotherapy Research, 2020: 1–36. https://doi.org/10.1002/ptr.6889

Melro E., Filipe A., Valente A.J.M.., Antunes F.E., Romano A., Norgren M., Medronho B., 2020. Levulinic acid: a novel sustainable solvent for lignin dissolution. International Journal of Biological Macromolecules, 164: 3454-3461. https://doi.org/10.1016/j.ijbiomac.2020.08.128

Jara-Palacios M.J., Gonçalves S., Heredia F.J., Hernanz D., Romano A., 2020. Extraction of antioxidants from winemaking byproducts: effect of the solvent on phenolic composition, antioxidant and anti-cholinesterase activities, and electrochemical behaviour. Antioxidants, 9: 675. https://doi.org/10.3390/antiox9080675

 Mariana Lopes; Chloé Pierrepont; Carla Margarida Duarte; Alexandra Filipe; Bruno Medronho; Isabel Sousa., 2020. "Legume Beverages from Chickpea and Lupin, as New Milk Alternatives". Foods, 9, 1458. https://doi.org/10.3390/foods9101458

Malin From; Per Tomas Larsson; Bo Andreasson; Bruno Medronho; Ida Svanedal; Håkan Edlund; Magnus Norgren., 2020. "Tuning the properties of regenerated cellulose: Effects of polarity and water solubility of the coagulation medium". Carbohydrate Polymers, 236, 116068-116068. https://doi.org/10.1016/j.carbpol.2020.116068

Yang, Jiayi; Medronho, Bruno; Lindman, Björn & Norgren, Magnus., 2020. Simple One Pot Preparation of Chemical Hydrogels from Cellulose Dissolved in Cold LiOH/Urea. Polymers (Basel), 12, 373. https://doi.org/10.3390/polym12020373

Magalhães S., Alves L., Medronho B., Romano A., Rasteiro M., 2020. Bio-based solutions for removal of microplastics in ecosystems: current trends and future perspectives. Molecules, 25(17), 3954. https://doi.org/10.3390/molecules25173954

Das G., Patra J.K., Gonçalves S., Romano A., Gutiérrez-Grijalva E.P., Basilio Heredia J., Talukdar A.D., Shome S. & Shin H-S., 2020. Galangal, the multipotent super spices: A comprehensive review. Trends in Food Science & Technology, 101: 50-62. https://doi.org/10.1016/j.tifs.2020.04.032

Coelho N., Gonçalves S. & Romano A., 2020. Endemic plant species conservation: biotechnological approaches. Plants, 9, 345; Review. [Q1 – Plant Science; IF= 2.632]. https://doi.org/10.3390/plants9030345

Melro E., Filipe A., Antunes F.E., Sousa D., Romano A., Valente A.J.M. & Medronho B., 2020. Dissolution of kraft lignin in alkaline solutions. International Journal of Biological Macromolecules, 148: 688-695. https://doi.org/10.1016/j.ijbiomac.2020.01.153

Aliaño-González, M.J.; Jarillo, J.A.; Carrera, C.; Ferreiro-González, M.; Álvarez, J.Á.; Palma, M.; Ayuso, J.; Barbero, G.F.; Espada-Bellido, E., 2020. Optimization of a Novel Method Based on Ultrasound-Assisted Extraction for the Quantification of Anthocyanins and Total Phenolic Compounds in Blueberry Samples (Vaccinium Corymbosum L.). Foods, 9, 1763. https://doi.org/10.3390/foods9121763

Aliaño-González, M.J.; Espada-Bellido, E.; Ferreiro-González, M.; Carrera, C.; Palma, M.; Ayuso, J.; Álvarez, J.Á.; F. Barbero, G., 2020. Extraction of Anthocyanins and Total Phenolic Compounds from Açai (Euterpe Oleracea Mart.) Using an Experimental Design Methodology. Part 2: Ultrasound-Assisted Extraction. Agronomy, 10, 326. https://doi.org/10.3390/agronomy10030326

Aliaño-González, M.J.; Ferreiro-González, M.; Espada-Bellido, E.; Barbero, G.F.; Palma, M., 2020. Novel Method Based on Ion Mobility Spectroscopy for the Quantification of Adulterants in Honeys. Food Control, 114, 107236. https://doi.org/10.1016/j.foodcont.2020.107236

Aliaño-González, M.J.; Ferreiro-González, M.; Espada-Bellido, E.; Carrera, C.; Palma, M.; Ayuso, J.; Barbero, G.F.; Álvarez, J.Á., 2020. Extraction of Anthocyanins and Total Phenolic Compounds from Açai (Euterpe Oleracea Mart.) Using an Experimental Design Methodology. Part 3: Microwave-Assisted Extraction. Agronomy, 10, 179. https://doi.org/10.3390/agronomy10020179

Aliaño-González, M.J.; Ferreiro-González, M.; Espada-Bellido, E.; Carrera, C.; Palma, M.; Álvarez, J.A.; Ayuso, J.; F. Barbero, G., 2020. Extraction of Anthocyanins and Total Phenolic Compounds from Açai (Euterpe Oleracea Mart.) Using an Experimental Design Methodology. Part 1: Pressurized Liquid Extraction. Agronomy, 10, 183. https://doi.org/10.3390/agronomy100(2018)3

Aliaño-González, M.J.; Richard, T.; Cantos-Villar, E., 2020. Grapevine Cane Extracts: Raw Plant Material, Extraction Methods, Quantification, and Applications. Biomolecules, 10, 1195. https://doi.org/10.3390/biom10081195

Guerrero, R.F.; Aliaño-González, M.J.; Puertas, B.; Richard, T.; Cantos-Villar, E., 2020. Comparative Analysis of Stilbene Concentration in Grapevine Shoots of Thirteen Vitis during a Three-Year Study. Industrial Crops and Products, 156, 112852. https://doi.org/10.1016/j.indcrop.2020.112852

Calle, J.L.; Ferreiro-González, M.; Aliaño-González, M.J.; F. Barbero, G.; Palma, M., 2020. Characterization of Biodegraded Ignitable Liquids by Headspace–Ion Mobility Spectrometry. Sensors, 20, 6005. https://doi.org/10.3390/s20216005

Piotr Konieczka, P.; Aliaño-González, M.J.; Ferreiro-González, M.; Barbero, G.F.; Palma, M., 2020. Characterization of Arabica and Robusta Coffees by Ion Mobility Sum Spectrum. Sensors, 20, 3123. https://doi.org/10.3390/s20113123

Putri, A.R.; Aliaño-González, M.J.; Ferreiro, M.; Setyaningsih, W.; Rohman, A.; Riyanto, S.; Palma, M., 2020. Development of a Methodology Based on Headspace-Gas Chromatography-Ion Mobility Spectrometry for the Rapid Detection and Determination of Patin Fish Oil Adulterated with Palm Oil. Arabian Journal of Chemistry, 13, 7524–7532. https://doi.org/10.1016/j.arabjc.2020.08.026

2019

Rodríguez-Solana R., Coelho N., Santos-Rufo A., Gonçalves S., Pérez-Santín E. & Romano A., 2019.  The Influence of in vitro gastrointestinal digestion on the chemical composition and antioxidant and enzyme inhibitory capacities of carob liqueurs obtained with different elaboration techniques. Antioxidants, 8(11), 563. https://doi.org/10.3390/antiox8110563

Costa; Medronho; Filipe; Mira; Lindman; Edlund & Norgren., 2019. Emulsion Formation and Stabilization by Biomolecules: The Leading Role of Cellulose. Polymers (Basel), 11, 1570. https://www.mdpi.com/2073-4360/11/10/1570

Singh, Poonam; Magalhães, Solange; Alves, Luis; Antunes, Filipe; Miguel, Maria; Lindman, Björn & Medronho, Bruno., 2019. Cellulose-based edible films for probiotic entrapment. Food Hydrocoll, 88, 68–74. https://doi.org/10.1016/j.foodhyd.2018.08.057

Magalhães S., Alves L., Medronho B., Romano A., Coelho J.F.J. & Norgren M., 2019. Brief overview on bio-based adhesives and sealants. Polymers, 11: 1685 (Artigo de revisão). https://doi.org/10.3390/polym11101685

Medronho B., Filipe A., Napso S., Rein D.M., Pereira R.F.P., de Zea Bermudez V., Romano A. & Cohen Y., 2019. Silk fibroin dissolution in tetrabutylammonium hydroxide aqueous solution. Biomacromolecules, 20: 4107-4116. https://doi.org/10.1021/acs.biomac.9b00946

Gonçalves S., Mansinhos I., Rodríguez-Solana R., Pérez-Santín E., Coelho N. & Romano A., 2019. Elicitation improves rosmarinic acid content and antioxidant activity in Thymus lotocephalus shoot cultures. Industrial Crops and Products, 137: 214-220. https://doi.org/10.1016/j.indcrop.2019.04.071

Gonçalves S., Moreira E., Andrade P.B, Valentão P. & Romano A., 2019. Effect of in vitro gastrointestinal digestion on the total phenolic contents and antioxidant activity of wild Mediterranean edible plants extracts. European Food Research and Technology, 245: 753-762. https://doi.org/10.1007/s00217-018-3197-y

Rodríguez-Solana R., Salgado J.M., Pérez-Santín E. & Romano A., 2019. Effect of carob variety and roasting on the antioxidant capacity, and the phenolic and furanic contents of carob liquors. Journal of the Science of Food and Agriculture, 99: 2697–2707. https://doi.org/10.1002/jsfa.9437

Aliaño-González, M.J.; Ferreiro-González, M.; Barbero, G.F.; Palma, M., 2019. Novel Method Based on Ion Mobility Spectrometry Sum Spectrum for the Characterization of Ignitable Liquids in Fire Debris. Talanta, 199, 189–194. https://doi.org/10.1016/j.talanta.2019.02.063

Aliaño-González, M.J.; Ferreiro-González, M.; Espada-Bellido, E.; Palma, M.; Barbero, G.F., 2019. Screening Method Based on Headspace-Ion Mobility Spectrometry to Identify Adulterated Honey. Sensors, 19, 1621. https://doi.org/10.3390/s19071621

Aliaño-González, M.J.; Ferreiro-González, M.; Espada-Bellido, E.; Palma, M.; Barbero, G.F., 2019. A Screening Method Based on Visible-NIR Spectroscopy for the Identification and Quantification of Different Adulterants in High-Quality Honey. Talanta, 203, 235–241. https://doi.org/10.1016/j.talanta.(2019).05.067

Ferreiro-González, M.; Amores-Arrocha, A.; Espada-Bellido, E.; Aliaño-Gonzalez, M.J.; Vázquez-Espinosa, M.; González-de-Peredo, A.V.; Sancho-Galán, P.; Álvarez-Saura, J.Á.; Barbero, G.F.; Cejudo-Bastante, C., 2019. Escape ClassRoom: Can You Solve a Crime Using the Analytical Process? Journal Chemical Education, 96, 267–273. https://doi.org/10.1021/acs.jchemed.8b00601

Martins, I.M., Meirinho, S., Costa, R., Cravador, A., Choupina, A., 2019. Cloning, characterization, in vitro and in planta expression of a necrosis-inducing Phytophthora protein 1 gene npp1 from Phytophthora cinnamomi. Molecular Biology Reports46(6), 6453–6462. https://doi.org/10.1007/s11033-019-05091-0

Nunes, F.D., Sousa, F.M.G., Leitão, J.M.N., 2019. Characterization and performance analysis of generalized BOC modulations for GNSS. Navigation, Journal of the Institute of Navigation, 66(1), 185–197. http://dx.doi.org/10.1002/navi.279

Fazenda, P., Pereira, R., Fonseca, M., Carlier, J., Leitão, J., 2019. Identification and validation of microsatellite markers in strawberry tree (Arbutus unedo L.). Turkish Journal of Agriculture and Forestry, 43(4), 430–436. https://doi.org/10.3906/tar-1807-164

2018

Alves, Luis; Medronho, Bruno; Filipe, Alexandra; E. Antunes, Filipe; Lindman, Björn; Topgaard, Daniel; Davidovich, Irina & Talmon, Yeshayahu., 2018. New Insights on the Role of Urea on the Dissolution and Thermally-Induced Gelation of Cellulose in Aqueous Alkali. Gels, 4, 87. http://www.mdpi.com/2310-2861/4/4/87

Singh, Poonam; Medronho, Bruno; Santos, Tiago dos; Nunes-Correia, Isabel; Granja, Pedro; Miguel, Maria G. & Lindman, Björn., 2018. On the viability, cytotoxicity and stability of probiotic bacteria entrapped in cellulose-based particles. Food Hydrocoll, 82, 457–465. https://doi.org/10.1016/j.foodhyd.2018.04.027

Melro, Elodie; Alves, Luis; Antunes, Filipe E. & Medronho, Bruno., 2018. A brief overview on lignin dissolution. J. Mol. Liq, 265, 578–584. https://doi.org/10.1016/j.molliq.2018.06.021

Singh, Poonam; Medronho, Bruno; Valente, Artur J. M.; Miguel, Maria G. & Lindman, Björn., 2018. Exploring the prebiotic effect of cyclodextrins on probiotic bacteria entrapped in carboxymetyl cellulose-chitosan particles. Colloids Surfaces B Biointerfaces, 168, 156–162. http://dx.doi.org/10.1016/j.colsurfb.2017.12.014

Singh, Poonam; Medronho, Bruno; Miguel, Maria G. & Esquena, Jordi., 2018. On the encapsulation and viability of probiotic bacteria in edible carboxymethyl cellulose-gelatin water-in-water emulsions. Food Hydrocoll, 75, 41–50. https://doi.org/10.1016/j.foodhyd.2017.09.014

Haffner, F. B.; Canilho, N.; Medronho, B.; Gardiennet, C.; Gansmüller, A. & Pasc, A., 2018. In situ follow-up of hybrid alginate–silicate microbeads formation by linear rheology. Phys. Chem. Chem. Phys, 20, 11819–11825. https://doi.org/10.1039/C8CP00073E

Medronho B., Filipe A., Costa C., Romano A., Lindman B., Edlund H. & Norgren M., 2018. Microrheology of novel cellulose stabilized oil-in-water emulsions. Journal of Colloid and Interface Science, 531: 225-232. https://doi.org/10.1016/j.jcis.2018.07.043

Rodríguez-Solana R., Galego L.R., Pérez-Santín E. & Romano A., 2018. Production method and varietal source influence the volatile profiles of spirits prepared from fig fruits (Ficus carica L.). European Food Research and Technology, 244: 2213-2229. https://doi.org/10.1007/s00217-018-3131-3

Aghraz A., Gonçalves S., Rodríguez-Solana R., Ait Dra L., Di Stefano V., Dugo G., Cicero N., Larhsini M., Markouk M. & Romano A., 2018. Antioxidant activity and enzymes inhibitory properties of several extracts from two Moroccan Asteraceae species. South African Journal of Botany, 118: 58-64. https://doi.org/10.1016/j.sajb.2018.06.017

Jara-Palacios M.J., Gonçalves S., Hernanz D., Heredia F.J. & Romano A., 2018. Effects of in vitro gastrointestinal digestion on phenolic compounds and antioxidant activity of different white winemaking byproducts extracts. Food Research International, 109: 433-439. https://doi.org/10.1016/j.foodres.2018.04.060

Pereira A., Duarte H., Nosrati P., Gubitosi M., Gentile L., Romano A., Medronho B. & Olsson U., 2018. Cellulose gelation in NaOH solutions is due to cellulose crystallization. Cellulose, 25: 3205–3210. https://doi.org/10.1007/s10570-018-1794-3

Gonçalves S., Medronho J., Moreira E., Grosso C., Andrade P.B., Valentão P. & Romano A., 2018. Bioactive properties of Chamaerops humilis L. - antioxidant and enzyme inhibiting activities of extracts from leaves, seeds, pulp and peel. 3 Biotech, 8: 88-96. https://doi.org/10.1007/s13205-018-1110-9

Rodríguez-Solana R., Carlier J.D., Costa M.C. & Romano A., 2018. Multi-element characterization of carob, fig and almond liqueurs by MP-AES. Journal of the Institute of Brewing, 124: 300–309. https://doi.org/10.1002/jib.495

Aliaño-González, M.J.; Ferreiro-González, M.; Barbero, G.F.; Ayuso, J.; Palma, M.; Barroso, C.G., 2018. Study of the Weathering Process of Gasoline by ENose. Sensors, 18, 139. https://doi.org/10.3390/s18010139

Aliaño-González, M.J.; Ferreiro-González, M.; Barbero, G.F.; Ayuso, J.; Álvarez, J.A.; Palma, M.; Barroso, C.G., 2018. An Electronic Nose Based Method for the Discrimination of Weathered Petroleum-Derived Products. Sensors, 18, 2180. https://doi.org/10.3390/s18072180

Aliaño-González, M.J.; Ferreiro-González, M.; Barbero, G.F.; Palma, M.; Barroso, C.G., 2018. Application of Headspace Gas Chromatography-Ion Mobility Spectrometry for the Determination of Ignitable Liquids from Fire Debris. Separations, 5, 41. https://doi.org/10.3390/separations5030041

Ferreiro-González, M.; Aliaño-González, M.J.; Barbero, G.F.; Palma, M.; Barroso, C.G., 2018. Characterization of Petroleum-Based Products in Water Samples by HS-MS. Fuel, 222, 506–512. https://do.org/10.1016/j.fuel.2018.02.127

Jung, T., Durán, A., Sanfuentes von Stowasser, E., .Maia, C., Horta Jung, M., 2018. Diversity of Phytophthora species in Valdivian rainforests and association with severe dieback symptoms. Forest Pathology, 48(5), 12443. https://doi.org/10.1111/efp.12443